Whole Slide Preview Image Segmentation and Setup for Digital Pathology Scanners

Abstract

The problem of tissue finding is of special interest in automating WSI scanners where it decomposes the preview image of tissue glass slides into a simplified and abstract level of localization and identification to setup WSI scanner for high-resolution scan. Prior to such scanning, a preview image is captured to calibrate the scanners parameters. Scan parameters such as focus depth and scan region are determined using a tissue finding software package. This paper introduces a series of pipelines (e.g. binary mask segmentation, tissue/artifact classification, region-of-interest allocation) to automate tissue preview segmentation in both brightfield and darkfield microscopy.