Roary: Rapid large-scale prokaryote pan genome analysis

Abstract

SummaryA typical prokaryote population sequencing study can now consist of hundreds or thousands of isolates. Interrogating these datasets can provide detailed insights into the genetic structure of of prokaryotic genomes. We introduce Roary, a tool that rapidly builds large-scale pan genomes, identifying the core and dispensable accessory genes. Roary makes construction of the pan genome of thousands of prokaryote samples possible on a standard desktop without compromising on the accuracy of results. Using a single CPU Roary can produce a pan genome consisting of 1000 isolates in 4.5 hours using 13 GB of RAM, with further speedups possible using multiple processors.\n\nAvailability and implementationRoary is implemented in Perl and is freely available under an open source GPLv3 license from http://sanger-pathogens.github.io/Roary\n\nContactroary@sanger.ac.uk\n\nSupplementary informationSupplementary data are available at Bioinformatics online.