Isolation and identification of Phytophthora species on nut-bearing plants

The paper describes the isolation and identification of pathogens from soil samples taken in the Moscow region from walnut plants with signs of wilting and stem ulcers. Isolation of Phytophthora from soil samples was carried out by the method of floating biological baits with subsequent isolation of pathogens on a semi-selective nutrient medium - P5ARP [H]. Samples were taken from the colonies for DNA isolation. After that, PCR analysis was carried out with primers YPh1F / YPh2R and ITS4 / ITS5 and sequencing. As a result of the studies carried out, colonies similar to the morphological characteristics of P. cactorum and P citricola were obtained and confirmed by sequencing.