Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers
J. Schmitz, B. Stute, S. Taeuber, D. Kohlheyer, E. von Lieres, A. Gruenberger
Abstract
We present a new microfluidic trapping concept to retain randomly moving suspension cells inside a cultivation chamber. In comparison to previously published complex multilayer structures, we achieve cell retention by a thin PDMS barrier, which can be easily integrated into various PDMS-based cultivation devices. Cell loss during cultivation is effectively prevented while diffusive media supply is still ensured.
02 /Specification & Classification
Click any tag to browse matching papers- Approach
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- A cost-effective way to design a two-layered cell trapping microfluidic device
J. M. Lazaro·bioRxiv·May 29, 2021
- Development of a single layer microfluidic device for dynamic stimulation, culture and imaging of mammalian cells
A. Mustafa, E. Pedone, … L. Marucci·bioRxiv·Mar 14, 2022
- Reversibly-bonded Microfluidic Devices for Stable Cell Culture and Rapid, Gentle Cell Extraction
X. FENG, L. K. W. Cheng, … A. R. Wu·bioRxiv·Dec 6, 2023
- Reconfigurable microfluidic circuits for isolating and retrieving cells of interest
C. Deroy, J. H. R. Wheeler, … E. J. Walsh·bioRxiv·Dec 23, 2021
- Agile manufacturing of PDMS microfluidic devices for cell culture without a clean room
Jean-Abel Merino, Cristian Angeli, Juan Manuel Garcia-Arcos·bioRxiv·Nov 9, 2026
- The VersaLive platform enables pipette-compatible microfluidic mammalian cell culture for versatile applications
G. M. Nocera, G. Viscido, … D. di Bernardo·bioRxiv·Jul 2, 2021