RNA-seq analysis of transcriptomes for assessing stress tolerance of S. cerevisiae strain, NCIM3186

Abstract

We have previously sequenced the draft genome of high ethanol producing S. cerevisiae strain, NCIM3186. Towards assessing the stress tolerance by this strain transcriptomes from control and in response to glucose, ethanol and furfural stress were sequenced. Comparative RNA-seq analysis of these transcriptomes identified 573 differentially expressed genes of which thiamine biosynthesis genes under furfural stress, TDH1, heat shock proteins and hexose transporter gene under ethanol stress were observed to be highly differentially expressed. Apart from thiamine biosynthesis genes and TDH1, 2 other proteins of unknown function were highly differentially expressed under glucose stress. Most importantly, TAR1 gene was highly down-regulated under all the stress conditions compared to control. Among 93 fermentome genes, 7 (TPS1, TPS2, SIN3, PTK2, SSQ1, ZAP1, DOA4) out of 9 stuck genes are found to be differentially expressed. Several stress-related genes like PHO4, SOD2, STR3, GRE2, GLR1, MEP1,3, MLH3, SNF1, MSN2, ATG1, GLC7 were differentially expressed.