Inferring biosynthetic and gene regulatory networks from Artemisia annua RNA sequencing data on a credit card-sized ARM computer

0.Prediction of gene function and gene regulatory networks is one of the most active topics in bioinformatics. The accumulation of publicly available gene expression data for hundreds of plant species, together with advances in bioinformatical methods and affordable computing, sets ingenuity as the major bottleneck in understanding gene function and regulation. Here, we show how a credit card-sized computer retailing for less than 50 USD can be used to rapidly predict gene function and infer regulatory networks from RNA sequencing data. To achieve this, we constructed a bioinformatical pipeline that downloads and allows quality-control of RNA sequencing data; and generates a gene co-expression network that can reveal enzymes and transcription factors participating and controlling a given biosynthetic pathway. We exemplify this by first identifying genes and transcription factors involved in the biosynthesis of secondary cell wall in the plant Artemisia annua, the main natural source of the anti-malarial drug artemisinin. Networks were then used to dissect the artemisinin biosynthesis pathway, which suggest potential transcription factors regulating artemisinin biosynthesis. We provide the source code of our pipeline and envision that the ubiquity of affordable computing, availability of biological data and increased bioinformatical training of biologists will transform the field of bioinformatics.\n\nHighlightsO_LIProcessing of large scale transcriptomic data with affordable single-board computers\nC_LIO_LITranscription factors can be found in the same network as their targets\nC_LIO_LICo-expression of transcription factors and genes in secondary cell wall biosynthesis\nC_LIO_LICo-expression of transcription factors and genes involved in artemisinin biosynthesis\nC_LI