Chelsea Y. Hu

Host-measured parts and inducible systems for Brucella melitensis ΔvjbR guided a modeled Cas9 kill-switch, which cut mouse liver bacterial burden by about 91% at 30 mg/kg doxycycline.

This paper develops a method to improve transformation efficiency in non-model Gram-negative bacteria by enhancing post-electroporation recovery.

The paper develops growth-aware optogenetic control strategies to maintain gene expression in bacterial batch cultures under population-level disturbances.

This paper describes the fabrication and use of a 32-well LED-embedded microplate (LEMOS 2.0) for optogenetic stimulation and gene expression measurement in microbial cultures.

This paper introduces LEMOS, a low-cost microplate reader for optogenetic feedback control in batch cultures, demonstrating closed-loop gene expression tracking and growth-aware controller design.

This paper develops a multiscale model, GEAGS, to predict transient oscillations in synthetic gene circuits by coupling gene expression to growth phases, revealing amino acid recycling and growth transitions as key factors.

This paper develops a field-deployable arsenic sensor using genetically modified Bacillus megaterium integrated with CMOS technology, achieving detection below EPA thresholds in soil and air.

This study demonstrates that layered feedback control in synthetic biomolecular networks in E. coli overcomes the robustness-efficiency trade-off, validating control theory predictions through experimental validation.

The paper develops a dynamical population model and type-1 IFFL controller to simulate and improve wound healing dynamics.

The paper designs and analyzes a layered feedback controller in synthetic biology, showing it combines the benefits of cis and trans feedback mechanisms.

This study designs and validates a small RNA-based negative autoregulation network using computational modeling and cell-free systems, showing reduced response time and noise in gene expression.