The Aedes aegypti bacterial microbiota is robust to infection with the obligate microsporidian parasite Edhazardia aedis.

Abstract

Edhazardia aedis is an obligate microsporidian parasite of the arthropod vector Aedes aegypti, which is responsible for the spread of several vertebrate pathogens of global health importance. E. aedis can be highly virulent to Ae. aegypti and infection has severely detrimental effects on multiple life history traits that are relevant to the vectoral capacity of Ae. aegypti, including longevity, body size, propensity to host-seek and blood-feed, and reproductive capacity. Because E. aedis is also highly specific to Ae. aegypti and is incapable of completing its full life cycle in any other mosquito species, E. aedis merits investigation as a novel tool for biological vector control. In the present study, we queried the effect of E. aedis infection on the bacterial microbiota of adult female Ae. aegypti using high-throughput amplicon sequencing of the 16S rRNA gene. Analysis of sequencing data revealed that the bacterial microbiota community is strikingly robust to E. aedis infection, as we observed no significant effect on alpha or beta diversity, differential abundance of any taxa, predicted metabolic function profile, or overall bacterial load. The data show that E. aedis, despite dramatically impacting the health and fitness of the adult female mosquito, does not affect the microbiota. These results provide unique insight into tripartite relationships (or lack thereof) between hosts, pathogens, and the microbiota.