bioRxivBiophysicsPreprint
SLICK: A Sandwich-LIke Culturing Kit for in situ Cryo-ET Sample Preparation
Qiuye Li, Li ZhangiD, Qin Lan XuiD, Peng ZhangiD, Shiwei ZhuiD
Abstract
In situ cryo-electron tomography (cryo-ET) has recently been widely used in observing subcellular structures and macromolecules in their native states at high resolution. One of the reasons that it has not been more widely adopted by cell biologists and structural biologists is the difficulties in sample preparation. Here we present the Sandwich-LIke Culturing Kit (SLICK), simplifying the procedure and increasing the throughput for sample preparation for in situ cryo-ET (69 words).
- Citations
- 2
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- ELI trifocal microscope: A precise cryogenic fabrication system to prepare target cryo-lamellae of cells for in situ cryo-ET study
- Automated cryo-lamella preparation for high-throughput in-situ structural biology
G. Buckley, G. Gervinskas, … A. de Marco·bioRxiv·Oct 8, 2019
- Fully automated, sequential focused ion beam milling for cryo-electron tomography
Tobias Zachs, Andreas Schertel, … Martin Pilhofer·eLifePeer-reviewed·Mar 9, 2020
- In-Chamber Sublimation: A Practical Approach for Mitigating Ice and Curtaining in Cryo-Electron Tomography Lamellae Preparation
Amy L. Bondy, Genís Valentín Gesé, … B. Martin Hällberg·bioRxiv·Mar 13, 2026
- VHUT-cryo-FIB, a method to fabricate frozen-hydrated lamella of tissue specimen for in situ cryo-electron tomography
- Making plant tissue accessible for cryo-electron tomography
Matthias Pöge, Marcel Dickmanns, … Wolfgang P. Baumeister·bioRxiv·Feb 14, 2025