Chromosome-level genome assembly of the spider mite Tetranychus cinnabarinus

Abstract

Tetranychus cinnabarinus is an important agricultural pest, primarily due to its rapid development of pesticide resistance. In this study, we constructed a chromosome-level genome of T. cinnabarinus using Illumina, Nanopore, and Hi-C sequencing technologies. The assembled genome spans 86.67 Mb, with an N50 value of 21.77 Mb, and 84.54 Mb of sequences anchored across three chromosomes. The BUSCO completeness of the genome is 92.78%. Through gene prediction, we identified 11,388 protein-coding genes, 98.53% of which are functionally annotated. Furthermore, we systematically identified 304 genes from five major detoxification enzyme systems. This high-quality genome provides a solid foundation for investigating the physiological mechanisms of T. cinnabarinus and for identifying potential targets for biocontrol strategies.