Wasabi leaf extract changes the expression levels of cytokine related genes in dermal papilla cells, which identified by the whole transcriptome

Abstract

Wasabi is a traditional food in Asian area. Wasabi leaf extract derived compounds such as 6-methylsulfinylhexyl isothiocyanate (6-MSITC) have been reported to exert anti-proliferative effects on cancer cells. The antibacterial action of wasabi leaf extract has been reported previously. However, the detailed biological effects of wasabi leaf extract are not fully understood. To get a clue for the understanding, we used next-generation sequencing to identify the upregulated or downregulated genes in immortalized human dermal papilla cells. Differentially expressed genes were identified in human papilla cells in the presence or absence of wasabi leaf extract. The effects of 6-MSITC and isosaponarin were evaluated. We found that wasabi leaf extracts upregulated or downregulated the expression of cytokine-related genes. The identified expression changes will contribute to our understanding of the biological effects of wasabi.