Mechanistic Study of palmatine in Regulating Pyroptosis in Sepsis Cells via Signaling Pathways

This study aims to investigate whether palmatine has a potential effect on sepsis-related pyroptosis in patients through relevant signaling pathways. Initially, the study explores the differential genes associated with pyroptosis in sepsis patients, along with their related enrichment processes, biological behaviors, and functional pathways. Patients with sepsis pyroptosis are then classified into subgroups based on pyroptosis-related genes to analyze the biological differences between these subtypes. Furthermore, the signaling pathways and biological functions associated with palmatine are studied to assess whether there are functional similarities or overlaps with the signaling pathways and biological behaviors observed in patients with sepsis pyroptosis. Finally, the findings are validated through wet-lab experiments. The results reveal that the signaling pathways related to palmatine partially overlap with those observed in sepsis pyroptosis patients, indicating that palmatine may influence sepsis pyroptosis through these pathways. Parmatine may improve the prognosis of sepsis by affecting the immune function of PRKACA, PTGS2, NLRP3, HSP90AA1 and PTPN22.