Laser Scanning Confocal printing, a new simple method for micro-device fabrication (LSCprint method).

Abstract

Micro-devices and lab-on-a-chip technologies have revolutionized cell biology research, enabling a plethora of applications from single-cell sorting to organ-on-a-chip assays. However, their construction remains laborious, requiring specialized equipment and trained personnel, thereby restricting their accessibility to specialized laboratories. The conventional protocol for micro-device printing involves intricate steps, including master cast/mold production and device fabrication, leading to high costs and time consumption. Here, we present a novel, simplified method utilizing a laser scanning confocal microscope (LSCM) and commercially available photosensitive resins. By using the UV or violet excitation laser lines of an LSCM, we eliminate the need for external suppliers and complex equipment, enabling any conventional cell biology laboratory to fabricate micro-devices swiftly and inexpensively. Our method not only enhances the capabilities of standard confocal microscopes but also democratises microfluidic device fabrication, making it accessible to non-specialized laboratories. With minimal reagent consumption and high scalability, our approach offers a cost-effective solution for rapid prototyping and production of micro-devices, circumventing previous barriers to widespread adoption. Moreover, our method allows direct printing of micro-devices onto substrates, eliminating the need for molds and intermediate steps, thus facilitating greater design flexibility and accessibility for non-specialized laboratories.