c-Fos protein shRNA blockade in the central amygdala nucleus interfere with rats emotional reactivity on behavioral and autonomic level

Abstract

This report is focusing on a function of the c-Fos protein in an associative, stress-induced memory. The shRNA vector injections were utilised to functionally silence the central amygdala nucleus in adult Wistar rats. Subsequently the operated animals and their control counterparts were screened in a selection of an emotionally-dependent tests and in a few standard behavioral neuroscience tools. Rats from the c-Fos silencing (ShFos) group expressed contra-depressive-like behaviors in Porsolt Swimming Test, spending more time actively searching for escape way then the rats from the control group. ShFos rats also had engaged in a more rapid activity in the Open Field Test, showing a decline in the neo-phobia. Micturition was decreased in shFos animals, indicating a change in the emotionality on an autonomic level. Presented results are showcasing a multi-directional regulation of the behaviors from the central amygdala nucleus by the c-Fos activity.