PanomiR: A systems biology framework for analysis of multi-pathway targeting by miRNAs

Abstract

Charting microRNA (miRNA) regulation across pathways is central to characterizing their role in disease. Yet, current methods reveal only individual miRNA-pathway interactions. We have developed a systems biology approach, Pathway networks of miRNA Regulation (PanomiR), that overcomes these limitations to identify miRNA targeting of groups of interacting pathways using gene expression. The approach does not depend on statistically significant enrichment of miRNA target genes in individual pathways or significant differentially expressed genes. Rather, it directly captures differential activity of pathways between states, determining their up-or-down regulation while sensitively detecting biologically-meaningful signals. PanomiR analyzes the co-activity of differentially regulated pathways to determine coordinate functional groups and uses these co-activated grouped pathways to uncover miRNAs that target them. Incorporating both experimentally-supported or predicted miRNA-mRNA interactions, PanomiR robustly identifies miRNAs central to the regulation of disease functions. We applied PanomiR to a liver cancer dataset and showed that it can organize liver cancer pathways and their regulating miRNAs into coordinated transcriptional programs, reflecting the pathogenic mechanisms of hepatocellular carcinoma. PanomiR recapitulated known central miRNAs in liver cancer with a biologically meaningful assignment of pathways under their regulation, unbiased by the number of genes targeted by each miRNA. PanomiR is a granular framework for detecting broad-scale multi-pathway programs under miRNA regulation. It is accessible as an open-source R/Bioconductor package: .