Novel Probe Design for Multi-Gene Detection Enabled by Cleavage and Capillary Electrophoresis

Abstract

Nucleic acid sequences carry the most fundamental information about life, and gene detection plays a crucial role in studying and applying gene function. Here, we present a novel probe design that utilizes probe cleavage and capillary electrophoresis for gene detection, termed ProbeCE which detects genes by measuring the relative fluorescence signal intensity. Through the evaluation of probes with varying lengths and modifications, ProbeCE is believed to support the simultaneous detection of hundreds of genes. Additionally, the assessment of low-concentration samples has demonstrated the high sensitivity of the ProbeCE method. Compared to the conventional amplification product capillary electrophoresis (AmpCE) method, this new approach significantly increases detection throughput while simplifying primer design and enhancing specificity. In addition, ProbeCE is less expensive than current medium- or high-throughput sequencing for detecting large numbers of known genes. GRAPHICAL ABSTRACT O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=85 SRC="FIGDIR/small/643483v1_ufig1.gif" ALT="Figure 1"> View larger version (19K): org.highwire.dtl.DTLVardef@188decborg.highwire.dtl.DTLVardef@dae443org.highwire.dtl.DTLVardef@c01a55org.highwire.dtl.DTLVardef@d653_HPS_FORMAT_FIGEXP M_FIG C_FIG