FixGrower: An efficient and robust curriculum for shaping fixation behavior in rodents

Abstract

Center-port fixation is a common prerequisite for many freely-moving rodent tasks in neuroscience and psychology. However, typical protocols for shaping this behavior are non-standardized and inefficient. Moreover, motor errors in fixation termed violations often account for a significant fraction of experimental trials, leading to notable data loss during experiments. In light of this, we developed FixGrower, a standardized protocol for center-port fixation training. FixGrower (1) requires a longer initial fixation requirement, (2) increases the required fixation duration at session boundaries customized to each animals performance, and (3) delays the introduction of violation penalties until the end of training. We demonstrate FixGrower decreases training time by 61%, yields low violation rates, and generalizes across rodent species and task difficulty. Moreover, the success of this curriculum is well supported by theories of operant conditioning and reinforcement learning. Our findings establish FixGrower as an efficient and broadly applicable curriculum for training fixation behavior in rodents, thereby accelerating training of many tasks in the field.