Study of application times gibberellic acid and 2,4-Dichlorophenoxyacetic acid in the plant regeneration from wheat haploid embryos in chromosome elimination method

Abstract

Wheat is one of the most important cereals, which is very valuable in food. Haploid plants are of particular importance in plant breeding. The wheat seeds produced in the crosses between wheat and maize in the chromosome elimination method without of endosperm and are immature embryo, to prevent the abortions haploid embryos, they must have embryo rescue. Increasing production of haploid plants from produced embryos can improve production efficiency. In this study, With attention their effects gibberellic acid and 2,4-Dichlorophenoxyacetic acid on growth, cell size and cell division, Their use in the production of wheat haploid plant were studied. There was a significant difference at level 1% between the not use and use of gibberellic acid in difference times in the production of haploid from embryos, So that the most haploid plant produced in the use of gibberellic acid in the 4 days after pollination. Also, the use of 2,4-Dichlorophenoxyacetic acid in tiller maintenance liquid culture medium was evaluated at times 48 and 72 hours after pollination. There were a significant difference between these treatments at the 1% level and the most was obtained for wheat haploid plant production with application of 2,4-Dichlorophenoxyacetic acid treatment for 72 hours. Highlightproduction of haploid plants plays an important role in wheat breeding. This technique is done to get doubled haploid and absolute homozygous plants in a very short duration of time.