Positively selected variants in functionally important regions of TLR7 in Alouatta guariba clamitans with yellow fever virus exposure in Northern Argentina

1In 2007-2009, a ma jor yellow fever virus (YFV) outbreak in Northern Argentina decimated the local howler monkey (Alouatta) population. We explored the relationship between Toll-like receptor (TLR) 7 and TLR8 gene variation and YFV susceptibility using samples from Alouatta individuals alive before the YFV outbreak, individuals that died during the outbreak, and individuals that survived the outbreak and are alive today. We measured genetic divergence between Alouatta YFV exposure groups and evaluated Alouatta-specific substitutions for functional consequences. We did not find different allele frequencies in the post-YFV exposure Alouatta group compared to the pre-exposure group. However, we identified three nonsynonymous variants in TLR7 in A. guariba clamitans. Two of these substitutions are under positive selection in functionally important regions of the gene. These unique coding differences in A. guariba clamitans may affect YFV resistance, but more work is necessary to fully explore this hypothesis.