Rapid and affordable generation of compensation beads for nanoscale flow cytometry

Abstract

With the introduction of dedicated nanoscale flow cytometers, the need for suitable compensation beads has emerged. Here, we present a rapid and cost-effective method to generate [~]100 nm antibody-binding compensation beads compatible with a wide range of antibody species for use in nanoscale flow cytometry. This approach may provide a practical interim solution until commercial alternatives become available.