Body coloration and mechanisms of colour production in Archelosauria: The case of deirocheline turtles

Animal body coloration is a complex trait resulting from the interplay of multiple colour-producing mechanisms. Increasing knowledge of the functional role of animal coloration stresses the need to study the proximate causes of colour production. Here we present a description of colour and colour producing mechanisms in two non-avian archelosaurs, the freshwater turtles Trachemys scripta and Pseudemys concinna. We compare reflectance spectra; cellular, ultra-, and nano-structure of colour-producing elements; and carotenoid/pteridine derivatives contents in the two species. In addition to xanthophores and melanocytes, we found abundant iridophores which may play a role in integumental colour production. We also found abundant dermal collagen fibres that may serve as thermoprotection but possibly also play role in colour production. The colour of yellow-red skin patches results from an interplay between carotenoids and pteridine derivatives. The two species differ in the distribution of pigment cell types along the dorsoventral head axis, as well as in the diversity of pigments involved in colour production, which may be related to visual signalling. Our results indicate that archelosaurs share some colour production mechanisms with amphibians and lepidosaurs, but also employ novel mechanisms based on the nano-organization of the extracellular protein matrix that they share with mammals.