Agarose Floor Technique: A Simple Scaffold-Free Method For 3D Cell Culture And Multicellular Tumor Spheroids Formation

To the editors To the editors REFERENCES 3D cell cultures can close the gap between in vitro experiments used for discovery and in vivo designs used for efficacy and safety assessment before proceeding to clinical studies 1. 3D multicellular tumor spheroids reflect better the tumor environment in terms of phenotypic heterogeneity, nutrient and oxygen gradients, intervascular domains, and micrometastases 2.\n\nThis letter presents the agarose floor technique to obtain single-cell suspensions from oral cancer cells in nonadherent conditions. SCC-9 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in culture plastic wares with nonadhesive surface. 150 mm dish are made of nonadhesive for cells by coating with 3.2% sterile agarose (Analytical Grade, Promega, Madison, WI, USA) thin films (5-8 mL/dish). This ...