Differences in Brain Microtubule Electrical Activity of the Hippocampus and Neocortex from the Adult Rat

Abstract

Microtubules (MTs) are essential cytoskeletal structures in neurons that generate electrical oscillations in the frequency range of mammalian brain waves. However, the role of these MT oscillations in brain function remains largely unknown. Here, we sought to gain insight into MT electrical oscillatory activity from different brain regions with specific functions, the hippocampus and the neocortex from the adult rat brain. We obtained local field potentials (LFP) from the frozen brain regions under non-depolarized (high external NaCl) and depolarized (high external KCl) saline solutions, observing spontaneous oscillations under both conditions. The electrical oscillations of the brain tissue had different amplitudes in the absence (0 mV) or presence (100 mV) of holding potential and were inhibited by the MT stabilizer paclitaxel. A frequency domain spectral analysis of the time records revealed the presence of two major peaks at approximately [~]38 Hz and [~]93 Hz in both preparations. However, the energy contribution of each peak was different in the hippocampus compared to neocortex. Coupled with our electron microscopy observations, these data suggest that rat brain MTs produce electrical oscillations with specific properties in the various regions of the mammalian brain, which could be partially related as their intra-axonal distributions. MT oscillations may be implicated in the wave coherence of brain activity, supporting their contribution to the concept of a brain central oscillator that drives its function.