Genomic analysis identifies Campylobacter concisus genomospecies 2 as a novel species and proposes the name Campylobacter oralis

Abstract

Campylobacter concisus comprises two distinct genomospecies (GS) based on core genome analysis. Here we conducted further analysis and demonstrated that C. concisus GS2 strains belong to a novel Campylobacter species. A total of 245 C. concisus genomes including 85 GS1 and 160 GS2 strains were analysed. DNA-DNA hybridization (DDH) values between strains of GS1 and GS2 C. concisus were from 42.8% to 66%. Average Nucleotide Identity (ANI) between GS1 and GS2 C. concisus strains were from 87.8% to 89.7%. The average GC contents of GS1 and GS2 C. concisus strains were 37.3% and 39.2% respectively. GS1 and GS2 specific genes have been identified. GS1 and GS2 C. concisus strains can also be differentiated based on core genome, 23S rRNA polymorphisms and protein profiles. Their morphology appears different under certain conditions. In conclusion, ANI and DDH values clearly support that C. concisus GS2 as a novel Campylobacter species, for which we propose the name Campylobacter oralis. The identification of C. concisus and C. oralis specific genes, along with differences in their protein profiles, morphology, core genome and 23S rRNA phylogeny, further support this classification.