Detection and Whole Genome of Japanese Encephalitis Virus Genotype III strains isolated from Indian Pig and Mosquito vector

Abstract

Japanese encephalitis virus(JEV) are globally prevalent as deadly pathogens in human and animals including pig, horse and cattle. Japanese encephalitis (JE) still remains as an important cause of epidemic encephalitis worldwide and exists in a zoonotic transmission cycle. Assam is one of the highly endemic state for JE in India. In the present study, to understand the epidemiological status of JE circulating in pigs and mosquito particularly in Assam, India, molecular detection of JEV and the complete genome sequencing of the JEV isolates from pig and mosquito were done. The complete genome analysis of two JEV isolates from pig and mosquito were revealed7 and 21 numbers of unique points polymorphism of nucleotide during alignment of the sequences with other available sequences, respectively. Phylogenetic analysis revealed that the isolates of present investigation were belongs to genotype III and closely related with the strains of neighbouring country China. This study represents the transboundary nature of the JEV genotype III circulation and maintained the same genotype through mosquito-swine transmission cycles.