Ancestral Musaceae karyotype reconstruction provides insights into chromosome evolution and bract coloration

The banana family (Musaceae) exhibits remarkable diversity in both karyotype structure and bract coloration, yet the evolutionary dynamics of chromosomes and the genomic and regulatory basis underlying color diversification remain poorly understood. Here, we present a telomere-to-telomere (T2T), gap-free genome assembly of Musa exotica, an ornamental species with brightly colored bracts occupying an early-branching lineage within sect. Callimusa (Musa L.). By integrating this high-quality genome with other available Musaceae genomes, we reconstruct the ancestral Musaceae karyotype (AMK) for the first time, inferring a haploid chromosome number of n = 17. Comparative genomic analyses reveal recurrent, highly complex lineage-specific inter-chromosome rearrangements across extant Musaceae lineages, leading to inferred stepwise reductions in chromosome number to n = 11, 10, and 9. Notably, closely related species share similar rearrangement patterns, suggesting conserved evolutionary trajectories shaped by lineage-specific structural remodeling. Strikingly, rearrangement-associated regions are enriched for functionally important genes, particularly structural genes (CHS and F3H) and regulatory transcription factors (MYB and bHLH) involved in the anthocyanin biosynthesis pathway. Integrative transcriptomic and regulatory analyses further demonstrate coordinated activation of anthocyanin biosynthetic genes (CHS, CHI, F3'5'H, and ANS) in bracts, with expression divergence largely decoupled from gene dosage and predominantly driven by transcriptional regulation. Co-expression analyses reveal extensive MYB- and bHLH-enzyme interactions, underscoring their central role in modulating pathway activity and bract coloration diversity. Collectively, our findings suggest a link between genome structural evolution to trait diversification, offering a refined framework for understanding genome evolution and phenotypic diversification in Musaceae and other monocots. SignificanceWe reconstructed the ancestral Musaceae karyotype and revealed extensive lineage-specific chromosome rearrangements underlying karyotype evolution. Rearrangement-associated regions are enriched for anthocyanin biosynthetic and regulatory genes, suggesting that genome structural evolution may have contributed to bract coloration diversification in Musaceae. Integrative transcriptomic analyses further indicate that variation in anthocyanin-mediated bract coloration is more closely associated with transcriptional regulation than with gene dosage alone.