bioRxivDevelopmental BiologyPreprint
Trans-scale live imaging of an E5.5 mouse embryo using incubator-type dual-axes light-sheet microscopy
Go Shioi, Tomonobu M. WatanabeiD, Junichi Kaneshiro, Yusuke AzumaiD, Shuichi OnamiiD
Abstract
We report our success in simultaneously tracking tissue formation and single-cell migration in a mouse embryo on embryonic day 5.5 for 12 h. The microscope, incubator, and observation protocols were comprehensively specialized and optimized to achieve this. The present system revealed new phenomena that had not previously been observed and promises to elucidate the mechanism of mouse embryonic development in the future.
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