Intraflagellar Transport Selectivity Occurs with the Proximal Portion of the Trypanosome Flagellum

Abstract

Intraflagellar transport (IFT) trains move bidirectionally along the doublet microtubules (DMTs) of the axoneme within the flagellum. In Trypanosoma brucei, IFT trains predominantly associate with four of the nine DMTs. Using high-resolution microscopy, we reveal how this selective association is put in place. IFT proteins form a ring surrounding the 9 DMTs on top of the transition fibres. Volume electron microscopy revealed densities along all DMTs in the proximal portion of the flagellum, exhibiting thinner, shorter profiles with branches absent in mature IFT trains. As the axoneme extends within the flagellar pocket, IFT trains are detected but are often positioned outside DMTs 3-4/7-8. After the axoneme exits the flagellar pocket, IFT trains localise exclusively to DMTs 3-4 and 7-8. Super-resolution and expansion microscopy demonstrated that IFT proteins follow the same distribution as the IFT-like densities. This suggests they represent IFT trains undergoing assembly and/or disassembly and reveals their unexpected ability to shift from one DMT to another. SummaryIn Trypanosoma brucei, intraflagellar transport (IFT) trains selectively associate with specific axonemal microtubules. Using advanced microscopy, this study reveals how this restriction occurs at the proximal portion of the flagellum during the assembly and/or disassembly of IFT trains.