The Histopathological Assesments of Effecting Fe and Zn Heavy Metals in Cerebrum and Cerebellum Tissues of Rats

Heavy metals left intensively to the environment cause different healthy problems on people by entering the food chain heavy metals. Our aim is to provide determinig effect of in tissues especially in central nervous system as a result of consuming cause foods included heavy metals and water. Five groups were constituded by using 35 adult male Wistar-Albino sexual rats. Hematoxylin-eosin (H&E) staining was applied to determine the histological sides of the damages of heavy metals in cerebrum and cerebellum tissues and effects of given juglone (5-hydroxy-1,4-naphthoquinone) for reducing these damages. Besides, immunohistochemical TUNNEL method was applied to determine DNA damages in cell. Density of damage in cerebrum of Fe and Fe+Juglone groups were higher than in the control group. Apoptotic cells number was decreased in the group consisting of juglone. There was little damage and less apoptotic cells in treated Zn group. In the cerebellum similar cerebrum results were obtained. There were much degeneration Fe and Fe+Juglone groups cerebellum. Apoptotic cells were increased Fe groups. Fe/600 ppm. doses heavy metals have created a toxic effect on the cerebrum and cerebellum tissues.