In vitro anti cancer, anti inflammatory and anti oxidant activity of Celery oil (Apium graveolens)-Myristic acid based microemulsion system: Characterization and Biomedical application

Abstract

Plant based compounds are frequently modified, developed into drug components to treat different types of cancer. Celery is one of the most used ingredients in daily life, exhibits potential medicinal properties and myristic acid exhibits anti microbial and inflammatory properties making it ideal candidate for combinational therapy. This study demonstrates the formulation of celery oil-myristic acid based microemulsion system with sequence of weight/volume of components to optimize stable microemulsion. the sequence of formulations were performed, optimized through pseudo ternary phase diagram and stability analysis, of which CMA ME 4 formulation were observed to transparent and stable. This CMA ME 4 was analyzed using DLS for particle size which is found to be 14.0{+/-}27.1 and PDI 0.34{+/-}0.46 through 5th day to 30th day. In vitro drug release study has been performed with stimulated physiological condition (pH 5.6) and stimulated tumor tissue (pH 7.4), the microemulsion was withdrawn in time intervals and the kinetic model is constructed to detect the behaviour of the drug release profile. Further, anti inflammatory, anti diabetic and anti obesity assays were performed to analyse the potentiality of CMA ME 4 microemulsion formulation, where the results exhibited from 80 to 90% radical scavenging and inhibition compared with the synthetic drugs. A good cytotoxic activity of CMA ME 4 microemulsion was determined by positive results of the MTT assay performed with HeLa with 80 to 90% cell death, MCF7 with 80 to 90% cell death and HUVECs was used to determine the toxicity level when the microemulsion interacts with the normal cell which is found to be 80 to 90% cell viability over time. These findings more suitably suggest that combination of natural oil with synthetic components can be effective in treatment against diseases.