Tracking crystallophore nucleating properties: setting-up a database for statistical analysis.

Abstract

In this article, the principle of a database aimed at facilitating the understanding of the unique protein nucleating properties of the Crystallophore is presented. A first analysis allows us to compare the efficiency of Tb-Xo4 with the new Lu-Xo4 variant, featuring improved phasing properties. Then, the concept of subset-of-interest is introduced to reveal potential antagonistic/synergistic effects between Tb-Xo4 and physico-chemical parameters of the crystallisation kits such as pH. The overall approach may be of interest for any studies working on solutions dedicated to improve the nucleating step in protein crystallization. TOC Graphic O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=129 SRC="FIGDIR/small/057596v1_ufig1.gif" ALT="Figure 1"> View larger version (29K): org.highwire.dtl.DTLVardef@351291org.highwire.dtl.DTLVardef@5620fcorg.highwire.dtl.DTLVardef@8fadb2org.highwire.dtl.DTLVardef@d4c208_HPS_FORMAT_FIGEXP M_FIG C_FIG SynopsisA database and associated representation tools are highlighted to understand nucleating properties of the crystallophore.