bioRxivMolecular BiologyPreprint
Capture and Purification of Plant Genomic DNA on a Readily-available Cellulose Matrix
Abstract
Whatman FTA (R)Cards are a fast and efficient method for capturing and storing nucleic acids but are cost-prohibitive for some researchers. We developed a method that substitutes readily-available cellulose-based paper and homemade washing buffer for commercial FTA (R)Cards and FTA (R)Purification Reagent. This method is suitable for long-term storage of DNA from many plant species, including Arabidopsis thaliana, prior to purification and PCR.\n\nMethod SummaryHere we report a low-cost method for long-term storage of plant genomic DNA on a readily available cellulose matrix.
- organism: Arabidopsis
- assay: whole-genome sequencing
- research focus: variant calling
- approach: experimental assay
- Citations
- 0
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