bioRxivImmunology
Single-cell analysis of intestinal immune cells during helminth infection
L. Ferrer-Font, P. Mehta, P. Harmos, A. Schmidt, K. M. Price, I. F. Hermans, F. Ronchese, G. Le Gros, J. U. Mayer
Abstract
Single cell isolation from helminth infected intestines has been notoriously difficult, due to the strong anti-parasite type 2 immune responses that drive mucus production, tissue remodeling and immune cell infiltration. Through the systematic optimization of a standard intestinal digestion protocol, we were able to isolate millions of immune cells from heavily infected tissues. Using this protocol, we validated many hallmarks of anti-parasite immunity and analyzed immune cells from the lamina propria and granulomas during helminth development, as well as acute and chronic worm infection.
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