Streptococcus pneumoniae augments circadian clock gene expression in zebrafish cells

Abstract

The circadian clock is a highly coordinated, cell-autonomous process that regulates the daily internal rhythms of biological organisms. Circadian clocks have been identified in various organisms, including mammals, invertebrates, cyanobacteria, and plants. The zebrafish (Danio rerio) is a practical model for studying the vertebrate circadian clock due to its small size, ease of manipulation, gene tractability, and direct light responsiveness. Several studies have revealed that bacterial, viral, and parasitic infections can impact the expression of circadian genes in mammals. While some evidence suggests that this may also be the case in zebrafish, no studies have investigated the direct effects of bacterial exposure on the zebrafish clock. Here, using zebrafish Z3 cells, we show that exposure to heat-killed Streptococcus pneumoniae (HK-Spn) can augment the expression of core repressive factors, including per1b, per2, per3, and cry1a. Further investigation demonstrated that HK-Spn induces the production of reactive oxygen species (ROS) in Z3 cells and that the addition of NAC, a ROS antioxidant, blocks Spn-mediated induction of per2, cry1a, and per3. Additionally, HK-Spn augmented the expression of tefa and tefb, an effect NAC suppressed. These results suggest the involvement of a ROS-dependent pathway in the augmentation of per2, cry1a, and per3 by HK-Spn. Moreover, the activation of tefa and tefb by HK-Spn represents promising new targets for further investigation of the regulation of these genes.