Epigenetic reprogramming guides sexual dimorphism during floral development in Silene latifolia

Abstract

Dioecy, the condition in which male and female individuals exist as separate plants, represents a fascinating and relatively rare reproductive strategy, offering unique opportunities to study the genetic and epigenetic regulation of sexual dimorphism. While sex determining genes underlying dioecy have already been described for several plant species, the role of epigenetic modifications in meristematic cell populations remains poorly understood. In this study we describe the spatio-temporal deposition of three epigenetic markers during early stages of floral development in model dioecious species Silene latifolia. We selected H3K4me1, H3K9me2 and active Ser2 phosphorylated form of RNA Polymerase II (Pol-IIS2ph), to assess levels of chromatin condensation and transcriptional activity of meristematic cells during key developmental stages. Utilizing the novel approach of an AI-assisted nuclei segmentation and high-content imaging we created a single-cell resolution atlas for male and female floral meristems. Our results show a relationship between transcription activity and sex determination during early meristem development. Moreover, our results suggest that H3K9me2 deposition in the developing meristem is linked to sex-specific chromatin reprogramming events, such as pollen mother cell formation during anther maturation. Overall, these results offer new insights into the role of chromatin regulation during floral meristem development and improves our understanding of sexual dimorphism in dioecious species.