Hana Polášek-Sedláčková

This study uses MINFLUX nanoscopy to directly visualize MCM helicase activation and replisome coupling in human cells, showing sister replisomes remain coupled at ~40 nm during S phase.

The paper identifies PAF15-PCNA assembly as a rate-limiting factor for lagging strand replication and replisome integrity, showing its regulation by the S-phase checkpoint.

This study identifies CRL4DCAF12 as a regulator of MCMBP that ensures proper DNA replication licensing by controlling nascent MCM complex assembly and stability.

This study investigates epigenetic markers during floral development in Silene latifolia, showing their role in sex determination and chromatin reprogramming.

This paper develops a cell-permeable Biotin-HaloTag ligand to enable sequential labeling and separation of distinct protein variants for functional studies.

The paper introduces a quantitative AI-based DNA fiber workflow to analyze replication stress, demonstrating its efficiency and robustness through independent experimental datasets.