Ultra-long stable biomimetic nanoparticle Click-ed-to-cancer membrane for anti-cancer treatment

Abstract

Cancer cell membrane coated biomimetic nanoparticles have been shown to be highly efficient in cellular uptake, homotypic tumour targeting, and the ability to suppress tumour growth compared to uncoated nanoparticles. Long duration anti-cancer treatment regimens require highly stable cancer cell membrane coated biomimetic nanoparticle. To manufacture such highly stable cancer cell membrane coated biomimetic nanoparticle, we used "Click-chemistry" to encapsulate cancer cell membrane on nanoparticles. In situ characterization was done to confirm the functionality of the novel Click-chemistry based formulation to encapsulate cancer cell membrane on nanoparticles. Gold nanoparticles were encapsulated with the cell membranes of cell lines of lung adenocarcinoma, malignant melanoma, high-grade serous epithelial ovarian cancer, colorectal cancer, oral cancer, esophageal adenocarcinoma, adenoid cystic carcinoma of salivary gland, and breast cancer. Functional group analysis, size, morphology, and surface charge confirmed long-stability of the biomimetic nanoparticles after incubating in complete growth medium for 12-months.