Eisuke Kanao

This paper introduces two modified CHAMP-N methods to extend N-terminal peptide length, improving N-terminome coverage while maintaining technical reproducibility.

The paper introduces DiReCT, a method to improve peptide solubility and recovery for nanoHILIC/MS/MS proteomics by integrating solubilization and elution in a single StageTip workflow.

This paper evaluates two SDB-containing PTFE disks for proteomics, finding one provides better peptide recovery, especially for low-input samples.

This study examines how RNA-protein coupling changes in osteocytes under hyperglycemic conditions, revealing post-transcriptional regulation of the proteome.

This paper introduces a desalting tip column (ChocoTip) that improves peptide recovery in proteomics, outperforming existing methods in sensitivity and coverage.

The paper develops a protein half-life prediction model, PLTNUM, using a protein language model trained on NIH3T3 mouse data, achieving 71% accuracy and identifying key sequence features affecting half-life.

This paper describes a method to coat living cell membranes onto nanoparticles, demonstrating improved biocompatibility and reduced phagocytosis.

This paper develops a high-speed LC/MS/MS system achieving 1,000 samples per day with high sensitivity and depth, demonstrating its application in spatial proteomics of mouse brain.