bioRxivGenomicsPreprint
Generating high quality reference genomes from field collected specimens by optimizing preservation
Jeremiah J. MinichiD, Malia L. MooreiD, Nicholas A. AllsingiD, Emily R. MurrayiD, Loi Tran, Todd P. MichaeliD
Abstract
Sample preservation challenges efforts to generate high-quality reference genomes or pangenomes for all 2 million+ plant and animal species. Here we show 95% ethanol protects against degradation for fish blood (22{degrees}C, [≤]6 weeks) and plant tissue (4{degrees}C, [≤]3 weeks). Using Nanopore, we assemble high quality reference genomes from three fish and two plant species (contig N50: 6.5-13.8Mb; BUSCO completeness: 94.4-99.2%; QV: 43.8 for M. esculenta).
- organism: plant (other)
- organism: multiple species
- assay: whole-genome sequencing
- research focus: genome assembly
- approach: experimental assay
- Citations
- 0
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