Contrasting effects of accumulation and tolerance characteristics in Arabidopsis thaliana under Cr(III) and Cr(VI) stress

Abstract

In this study, for the first time we investigated Cr(III) and Cr(VI) stress-induced physiological and biochemical responses in Arabidopsis thaliana. The capacity of A. thalian to accumulate Cr is closely related to the valence of chromium. Cr(VI) was more toxic than Cr(III) as indicated by chromium accumulation and growth inhibition. When the concentration of chromium is greater than 200M, the root length and biomass of A. thaliana are reduced. But interestingly, Cr(III) at 200M increased the root length and biomass of A. thaliana compared to the control. The transmission electron microscope shows that Cr(VI) can cause the chloroplasts damaged and the chlorophyll reduced more than Cr(III). The chloroplasts were filled the starch grains. An increase of lipid peroxidation in A. thaliana roots caused by Cr was measured, and this effect increases as the increasing Cr. It indicated that A. thaliana suffers from Cr-induced oxidative stress which resulted cell death in roots. To fight against oxidative stress, Ascorbate peroxidase and Glutathione reductase were activated by Cr in antioxidant defense. The inhibition of growth, the accumulation of chromium, the responses of antioxidant systems, and the ultra-morphological changes indicate that Cr(VI) was more toxic than Cr(III).