The P25 protein of Potato virus X suppresses accumulation of 24nt siRNAs in the N. benthamiana transgenic line 16c

Abstract

By transiently expressing viral suppressors of RNA silencing (VSRs) in combination with GFP silencing-inducing constructs in leaves of Nicotiana benthamiana line 16c, Hamilton et al. (2002) could establish a positive correlation between the production of 24nt GFP small interfering (si)RNAs in infiltrated leaves and the systemic onset of GFP silencing in remote tissues. In the context of GFP silencing inducers based on replicating Potato virus X (PVX), the P25 protein of PVX was found to specifically inhibit the local accumulation of 24nt GFP siRNAs. In the original paper, there were background pixel pattern duplications in the figure reporting the P25 experiments. We have now repeated these experiments with the original clones and the results presented here confirm those reported in the original paper.