Transcriptome analysis of the necrotrophic pathogen Alternaria brassicae reveals a biphasic mode of pathogenesis in Brassica juncea

Abstract

Alternaria blight or leaf spot caused by Alternaria brassicae has an enormous economic impact on the Brassica crops grown worldwide. Although the genome of A. brassicae has been sequenced, little is known about the genes that play a role during the infection of the host species. In this study, the transcriptome expression profile of A. brassicae during growth and infection was determined. Differential expression analysis revealed that 3921 genes were differentially expressed during infection. Weighted gene co-expression network analysis helped identify nine modules, which were highly correlated with growth and infection. Subsequent gene ontology (GO) enrichment analysis of the modules highlighted the involvement of biological processes such as toxin metabolism, ribosome biogenesis, polysaccharide catabolism, copper ion transport, and vesicular trafficking during infection. Additionally, 194 CAZymes and 64 potential effectors were significantly upregulated during infection. Furthermore, 17 secondary metabolite gene clusters were also differentially expressed during infection. The clusters responsible for the production of Destruxin B, Brassicicene C, and HC-toxin were significantly upregulated during infection. Collectively, these results provide an overview of the critical pathways underlying the pathogenesis of A. brassicae and highlight the distinct gene networks that are temporally regulated, resulting in a biphasic mode of infection. The study thus provides novel insights into the transcriptional plasticity of a necrotrophic pathogen during infection of its host. Additionally, the in planta expression evidence for many potential effectors provides a theoretical basis for further investigations into the effector biology of necrotrophic pathogens such as A. brassicae.