Context-dependent oviposition reveals strong association between acceptance and preference in the Mediterranean fruit fly

Abstract

Oviposition behavior in phytophagous insects is influenced by different stimuli and plays a key role in pest dynamics and crop loss. This study used 3D-printed artificial fruits varying in colour (yellow, blue, white) and odour (cherry, orange, banana) to test how visual and olfactory cues affect oviposition acceptance (no-choice) and preference (choice). In no-choice assays, the nine artificial fruits displayed sufficiently different visual and olfactory cues to trigger different egg-laying outputs (by a factor 1:3 between the least attractive fruit, white fruit with banana scent and the most attractive fruit, yellow fruit with cherry scent). While cues acted independently in no-choice settings, significant interactions were observed in choice conditions, highlighting multimodal sensory integration. In choice assays, the number of eggs laid and female preference depended on both the characteristics of fruits and their context. However a strong correlation was found between acceptance and preference. The relationship found between acceptance and preference implied that when a fruit seemed preferred in no-choice assays, it was even more preferred in one-choice assays. We finally discussed the practical implications for behavior-based pest management strategies.