MALDI-FISH for co-localization of brominated metabolites and Pseudovibrio spp. in Aplysina tissue

Abstract

Bromotyrosine-containing natural products have long been isolated from marine sponges of the order Verongiida. Recent studies have questioned the source of these natural products whether production occurs through the sponge, its diverse microbiota, or perhaps a combination of both. While studying the uptake of engineered bacterial strains by sponges, we have observed production of fistularin-3 in sponge. To explore these results further, we employed multimodal imaging techniques to co-localize microbial metabolites with fluorescent in situ hybridization (FISH) probes. Here, we built on previously developed MALDI-FISH methods to add to the conversation on brominated metabolite production in marine sponges. Using MALDI-MSI, we measured fistularin-3, a poly-brominated natural product previously isolated from Aplysina aerophoba, present in our A. aerophoba samples that were inoculated with Pseudovibrio brasiliensis. A recent study reported that P. brasiliensis produced fistularin-3 in pure culture, but this data has not yet been reproduced. We employed MALDI-MSI and FISH on the same sponge cryosections to colocalize the spatial distribution of fistularin-3 with P. brasiliensis in sponge tissue. Despite the caveats of this study, our data suggests that perhaps both sponge and microbe may be required for production of fistularin-3.