Transcriptomic Analysis Reveals MSTN Mutations and Mechanisms of Muscle Hypertrophy in a New Guinea Pig Breed

Abstract

The guinea pig (Cavia porcellus), a species native to Peru, is valued for its meat production, where muscle development is essential for productive efficiency. The new Kuri breed, obtained through selective breeding and genetic selection, has shown a phenotype with more developed musculature compared to native guinea pigs. In this study, we conducted a comparative transcriptomic analysis between Native and Kuri breed guinea pig, complemented by histological analysis of the semitendinosus muscle to investigate the underlying mechanisms responsible for the differences in muscle morphology. Histological analysis revealed a significant increase in muscle fiber area in the Kuri breed compared to the native guinea pigs. At the molecular level, key mutations were identified in the MSTN gene, including variants in the 3 UTR region and a frameshift mutation, which alter the genes inhibitory function on muscle growth. Additionally, differences were observed in the expression of pathways related to muscle degradation, energy metabolism, and angiogenesis, which explain the greater muscle hypertrophy in the Kuri breed. These findings provide a first understanding of the genetic mechanisms responsible for muscle hypertrophy in the Kuri breed and suggest candidate genes for improving meat quality through molecular genetic breeding programs in guinea pigs. AUTHOR SUMMARYThe guinea pig in Peru is valued for its meat production. The new Kuri breed, obtained through selective breeding and genetic selection has shown a phenotype with more developed musculature compared to other breeders. In our study, comparative transcriptomic analysis between Native and Kuri breed guinea pig, complemented by histological analysis of the semitendinosus muscle to investigate the underlying mechanisms responsible for the differences in muscle morphology. Histological analysis showed significant increase in muscle fiber area in the Kuri breed compared to the native. Furthermore, it is the first time that we identify in guinea pig, key mutations in the MSTN gene, which alter the genes inhibitory function on muscle growth. The impact of this finding will allow us to plan genetic improvement strategies in this species that promote muscle hypertrophy, paving the way for future research and its potential impact on guinea pig production.