Fecal contamination and water quality

Fecal Contributors to an Urbanized Estuary Identified by a Multi-Species qPCR Approach

This study uses multi-species qPCR to identify human and animal fecal sources in Mobile Bay, finding human waste as a major contributor.

Freshwater Microbiomes Shape Viral Inactivation in Continuous Cultures

The paper investigates how freshwater microbial communities influence viral inactivation, finding that specific bacterial taxa, not overall diversity, drive faster viral removal in lake-derived communities.

Distinct kinetics and mechanisms of microbial inactivation of enteric virus revealed by capsid and genome integrity

The paper investigates the inactivation kinetics and mechanisms of three enteric viruses in lakewater, showing differences in their decay rates and the role of microbial proteases and capsid integrity.

An Integrated Cell Culture - Nanopore Sequencing (ICC-NanoporeSeq) method for the simultaneous detection of multiple enterovirus types and quantification of infectious Enterovirus B

The paper develops ICC-NanoporeSeq to detect and quantify infectious Enterovirus B types in environmental samples, enabling type-resolved infectivity assessment.

A Capsid Tyrosine Residue Governs the Proteolytic Inactivation of Echovirus 11 in Lakewater

The paper identifies a single capsid residue in Echovirus 11 that determines its susceptibility to proteolytic inactivation in freshwater.

Systematic Comparison of Indicator and Pathogenic Viruses Using High-Throughput qPCR Identifies Pepper Mild Mottle Virus as a Robust Indicator of Virus Removal in Wastewater Treatment

The paper compares viral indicators and pathogenic viruses in wastewater treatment using qPCR, finding PMMoV as a reliable indicator due to its stability and consistent removal.

Differential Effects of Zooplankton on Sunlight Inactivation of Viruses

This study examines how zooplankton affect virus inactivation under sunlight and darkness, finding species-specific impacts on virus removal rates.

Simultaneous detection of protozoa, bacteria, and viruses from environmental water through membrane-adsorption followed by direct nucleic acid extraction

This paper develops a method for detecting protozoa, bacteria, and viruses in water using membrane adsorption and direct nucleic acid extraction, showing improved sensitivity for several pathogens.

Performance of Reasoner's 2 Agar (R2A) medium in enumeration of culturable microorganisms in water intended for human consumption

This study evaluates the R2A medium's performance for culturable microorganism enumeration in drinking water, recommending spread plate inoculation and 7-day incubation at 22°C.

Microbial source tracking of human and animal fecal contamination in Ecuadorian households

The paper uses microbial source tracking markers to identify human and animal fecal contamination in Ecuadorian households, finding that caregiver hand contamination is linked to child contamination.

MicrobiomePrime: A primer pair selection tool for microbial source tracking validated on a comprehensive collection of animal gut and fecal waste microbiomes

MicrobiomePrime identifies primer pairs for microbial source tracking by analyzing amplicon sequences, achieving high specificity in laboratory validation.

In-situ calibration of passive samplers for monitoring host-associated fecal markers in an urban river

This study evaluates two passive samplers for detecting fecal pollution markers in an urban river, showing they accumulate significantly more target genes than grab samples.

How flagella glycosylation of the phytopathogenic bacteria Pseudomonas amygdali pv. tabaci 6605 affect transport and deposition in saturated sandy porous media

This study examines how glycosylation of flagella in Pseudomonas amygdali pv. tabaci 6605 affects bacterial transport and deposition in sandy porous media through experiments and simulations.

Optimizing Digital PCR Assays for Multiplexed Microbial Source Tracking in Surface Waters

This paper optimizes triplex digital PCR assays for microbial source tracking in surface waters, showing comparable results to single-plex assays.

Rapid microbiology instrumentation for high-accuracy quantitative water safety assessments in remote and low-resource locations

This paper evaluates ALERT, a rapid microbiology instrument, as a field-deployable alternative to traditional methods for quantifying fecal indicator bacteria in water.

Comparative wastewater virome analysis with different enrichment methods

This study compares five viral concentration methods in wastewater to identify which best capture diverse viral taxa for metagenomic analysis.

Monitoring mammals, birds and fish during summer 2022 at the outlet of an agricultural stream by mtDNA

The paper monitors mammals, birds, and fish in an agricultural stream using mtDNA sequencing to identify sources of fecal contamination.

Microbial source tracking using metagenomic analysis from Cochin Estuary

This study uses metagenomic analysis to identify microbial pollution sources in the Cochin Estuary, finding sewage and domestic waste as major contaminants.

Evaluating the Survival and Removal of Escherichia coli from Surfaces Made with Traditional and Sustainable Cement-Based Materials in Field-Relevant Conditions

This study evaluates E. coli survival and removal on traditional and sustainable cement-based surfaces under field conditions, finding similar performance between fly ash and traditional mixes.

Lognormal distributions capture site-specific variability in enteric virus concentrations in wastewater

This study shows lognormal distributions accurately model enteric virus concentrations in wastewater, outperforming other distributions, and suggests weekly monitoring for effective and cost-effective tracking.

Addressing Underestimation of Waterborne Disease Risks Due to Fecal Indicator Bacteria Bound in Aggregates

The paper demonstrates that aggregate-bound fecal indicator bacteria are significantly undercounted by traditional methods, leading to underestimated waterborne disease risks.

Design and testing of a PCR assay to quantify coliform bacteria in drinking water

The paper develops and tests a qPCR assay targeting lacZ to quantify coliform bacteria in drinking water, achieving detection down to 1.82 x 103 cfu/100mL.

Advancing source tracking: systematic review and source-specific genome database curation of fecally shed prokaryotes

The paper curates nine genomic databases for fecal source tracking, summarizes their source-specificity, and tests their utility with mock water samples.

Impact of an Urban Sanitation Intervention on Enteric Pathogen Detection in Soils

This study evaluates the impact of a sanitation intervention on enteric pathogen detection in soils in Maputo, finding a 15% reduction in pathogen presence but persistent contamination.

Predicting E. coli concentrations using limited qPCR deployments at Chicago beaches

This paper develops a model using limited qPCR data and beach correlations to improve E. coli prediction at Chicago beaches, increasing sensitivity by 230%.