Efficient immune cell genome engineering with improved CRISPR editing tools
Waipan Chan, R. A. Gottschalk, Y. Yao, J. Pomerantz, R. N. Germain
Doxycycline-inducible dual-color Cas9, purified lentivirus, puromycin-selected nickase HDR, integrase-deficient lentiviral HDR delivery and an all-in-one CRISPRa vector enable editing of macrophage and Jurkat T cells.
bioRxiv · Feb 13, 2020 · PreprintGenomics✦ In-depth digest