A single nucleotide change in the polC DNA polymerase III in Clostridium thermocellum is sufficient to create a hypermutator phenotype
A. A. Lanahan, K. Zakowicz, L. Tian, D. G. Olson, L. R. Lynd
The paper introduces a C669Y mutation in polC of C. thermocellum, resulting in a 30-fold increase in mutation rate and a hypermutator phenotype.
bioRxiv · Jul 30, 2021Bioengineering