A Substrate-triggered μ-Peroxodiiron(III) Intermediate in the 4-Chloro-L-Lysine-Fragmenting Heme-Oxygenase-like Diiron Oxidase (HDO) BesC: Substrate Dissociation from, and C4 Targeting by, the Intermediate
M. J. McBride, M. A. Nair, D. Sil, J. W. Slater, M. E. Neugebauer, M. Chang, A. Boal, C. Krebs, J. M. Bollinger
The paper characterizes a substrate-triggered μ-peroxodiiron(III) intermediate in the enzyme BesC, showing it initiates oxidative fragmentation by abstracting hydrogen from C4 and allowing substrate dissociation.
bioRxiv · Dec 2, 2021Biochemistry