High yield preparation of outer-membrane protein efflux pumps by in vitro refolding is concentration dependent
S. J. Budiardjo, A. P. Ikujuni, E. Firlar, A. Cordova, J. Kaelber, J. S. G. Slusky
This study develops a concentration-dependent refolding protocol for outer-membrane efflux pump proteins, enabling high-yield preparation and structural analysis.
bioRxiv · Sep 14, 2020Biophysics